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it will,
above i think 75 degrees, most stuff is killed off, but I would be surprised if you probe your food on a regualr basis to ensure a core temperature.
The nastiest nasties are spore forming, so that instead of dying during heat, they sort of turn in to a seed, and then when temperature is right again, like down in your colon they just kick off. I studied a case while doing my advanced hygiene course on a boy that died from food he ate nearly 40 days earlier.

Edit: 75 degrees for 3 minutes is what we use, which i believe is above the legal minimum.