
Generation of dead flies
Dead flies were generated using starvation. Briefly, approximately 30 Canton S flies (of both sexes and 1 to 2 weeks of age) were placed into vials that contained 2% agar + 0.3% Tegosept media. The flies were placed in an 25°C incubator with 12:12 h light-dark cycles. Dead flies used for the dead-exposed treatments were collected for experimental use within 3 days of their death (termed “freshly dead”).
( , Wed 14 Jun 2023, 19:56, Reply)

'Flies were hit with a rolled up magazine and scraped off on the side of the settee.'
( , Wed 14 Jun 2023, 22:10, Reply)