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(I helped save b3ta! rob, Sun 1 Apr 2001, 1:00)
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DMSO and DMS for the minging win.
Although we used to grow a Strep strain that needed sheep blood meal in its media, that was fucking vile.
(I helped save b3ta! the mighty badger Aphrodite, on a bar stool, by your side, Tue 22 Nov 2011, 11:12, 1 reply, 14 years ago)
I've never found DMSO to be that bad
2-ME or TEMED, however...
(berk, Tue 22 Nov 2011, 11:13, Reply)
2-YOU?

(I helped save b3ta! Agnostic Antichrist Baltimora, Tue 22 Nov 2011, 11:14, Reply)
2-mercaptoethanol
it honks. Honks.
(berk, Tue 22 Nov 2011, 11:15, Reply)
I used to fucking hate running reduced gels because of that.

(I helped save b3ta! the mighty badger Aphrodite, on a bar stool, by your side, Tue 22 Nov 2011, 11:16, Reply)
Oof, not good.

(I helped save b3ta! Agnostic Antichrist Baltimora, Tue 22 Nov 2011, 11:16, Reply)
Oh, sweet jesus, mercaptoethanol, I'd actually erased that from my mind it's so fucking bad.
Cheers for that.

DMSO on its own is bearable but if you get anoxic transformation into DMS then it's fucking evil.
(I helped save b3ta! the mighty badger Aphrodite, on a bar stool, by your side, Tue 22 Nov 2011, 11:15, Reply)
We only ever use it for freezing cells down
so none of that, I don't think.
(berk, Tue 22 Nov 2011, 11:15, Reply)
yeah, some cells will do that transformation
so when you defrost them .... it's mingtown.
(I helped save b3ta! the mighty badger Aphrodite, on a bar stool, by your side, Tue 22 Nov 2011, 11:17, Reply)

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