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From the The iPad challenge. See all 368 entries (closed)
( , Thu 28 Jan 2010, 13:59, archived)

That's what it was like talking to him.
"Y'see, bruv - it's like we's da shadowz on da wall innit, bruv, an dat reality is jus an illusion wot we iz constantly facin, my bruvver ..."
( ,
Thu 28 Jan 2010, 14:06,
archived)
"Y'see, bruv - it's like we's da shadowz on da wall innit, bruv, an dat reality is jus an illusion wot we iz constantly facin, my bruvver ..."

I'd never heard it before and I honestly thought I'd ripped something I laughed so much.
( ,
Thu 28 Jan 2010, 14:14,
archived)

A new fluorophor for tubulin which has permitted the monitoring of microtubule assembly in vitro is reported. DAPI (4',6-diamidino-2-phenylindole), a fluorophor already known as a DNA intercalator, was shown to bind specifically to a unique tubulin site as a dimer (KD(app) = 43 +/- 5 microM at 37 degrees C) or to tubulin associated in microtubules (KD(app) = 6 +/- 2 microM at 37 degrees C) with the same maximum enhancement in fluorescence. When tubulin polymerization was induced with GTP, the change in DAPI affinity for tubulin resulted in an enhancement of DAPI binding and, consequently, of fluorescence intensity. DAPI, whose binding site is different from that of colchicine, vinblastine, or taxol, did not interfere greatly with microtubule polymerization. It induced a slight diminution of the critical concentration for tubulin assembly due to a decrease in the depolymerizing rate constant. Moreover, DAPI did not interfere with GTP hydrolysis correlated with tubulin polymerization, but it decreased the GTPase activity at the steady state of tubulin assembly. Even at substoichiometric levels DAPI can be used to follow the kinetics of microtubule assembly innit.
( ,
Thu 28 Jan 2010, 14:10,
archived)

that kind of sweet talk always gets me aroused
( ,
Thu 28 Jan 2010, 14:14,
archived)


I met a traveller from an antique land
Who said: "Two vast and trunkless legs of stone
Stand in the desert. Near them on the sand,
Half sunk, a shattered visage lies, whose frown
And wrinkled lip and sneer of cold command
Tell that its sculptor well those passions read
Which yet survive, stamped on these lifeless things,
The hand that mocked them and the heart that fed.
And on the pedestal these words appear:
`My name is Ozymandias, King of Kings:
Look on my works, ye mighty, and despair!'
Nothing beside remains. Round the decay
Of that colossal wreck, boundless and bare,
The lone and level sands stretch far away".
( ,
Thu 28 Jan 2010, 14:20,
archived)
Who said: "Two vast and trunkless legs of stone
Stand in the desert. Near them on the sand,
Half sunk, a shattered visage lies, whose frown
And wrinkled lip and sneer of cold command
Tell that its sculptor well those passions read
Which yet survive, stamped on these lifeless things,
The hand that mocked them and the heart that fed.
And on the pedestal these words appear:
`My name is Ozymandias, King of Kings:
Look on my works, ye mighty, and despair!'
Nothing beside remains. Round the decay
Of that colossal wreck, boundless and bare,
The lone and level sands stretch far away".

without having to look it up. It is a sad day for me.
edit 1: now I get why you said that. Apparently looking at the pic for more than 0.2 seconds was too much for me at first.
edit 2: but there ain't no whales so we tell tall tales and sing a whaling tune.
( ,
Thu 28 Jan 2010, 14:38,
archived)
edit 1: now I get why you said that. Apparently looking at the pic for more than 0.2 seconds was too much for me at first.
edit 2: but there ain't no whales so we tell tall tales and sing a whaling tune.

Is that how you become rich and famous these days?
( ,
Thu 28 Jan 2010, 14:07,
archived)

They have "been going" for 10 years apparently.
/read it in the paper the other day blog
But they have been trying to break america but can't get in as all 3 of them have a criminal record.
( ,
Thu 28 Jan 2010, 14:09,
archived)
/read it in the paper the other day blog
But they have been trying to break america but can't get in as all 3 of them have a criminal record.

I can't remember.
It was in the Sun. I was in a pub. Papers in pubs get read.
( ,
Thu 28 Jan 2010, 14:14,
archived)
It was in the Sun. I was in a pub. Papers in pubs get read.

Apart from the rich bit.
Oh, and the famous bit.
But the 'being a twat' bit still animates my universe.
(Which is the kind of thing a twat might say. See?)
( ,
Thu 28 Jan 2010, 14:14,
archived)
Oh, and the famous bit.
But the 'being a twat' bit still animates my universe.
(Which is the kind of thing a twat might say. See?)

*this is all, in fact, complete lies

Unless you work in a five star hotel, how could you possibly ever bump into Bear Grylls?
( ,
Thu 28 Jan 2010, 14:19,
archived)
